Fruit rot caused by Aspergillus niger or Colletotrichum musae is an important post-harvest disease on orange, chilli and Cavendish banana fruits. The use of synthetic fungicides has been a traditional strategy for the management of the fruit rot disease, but these chemicals adversely affect human health and environment. Therefore, the objective of this study was to evaluate the effects of CaCl₂ on in vitro hyphal growth and in vivo lesion inhibition. First, aqueous solutions of CaCl₂ at three concentrations of 20, 40 and 60 mM were assessed for their inhibitory effect against hyphal growth in vitro. Next, mature fruits were immersed into a solution of 20 mM CaCl₂ for 20 - 30 s, then inoculated by a pathogen suspension at the density of 106 conidia mL^-1 and observed for 12 days. The results showed that 20 mM CaCl₂ was the most effective concentration in antifungal assay to Aspergillus isolated from orange rot. The treatment of CaCl₂ continued to gain efficacy on limiting lesions’ development on orange fruits until 12 days after inoculation (DAI). On chilli, CaCl₂ at concentrations of 20 and 40 mM inhibited well on the growth of Aspergillus hyphae isolated from chilli rot. However, calcium treatment was not effective on chilli fruits. On Cavendish banana, solutions of CaCl₂ at concentrations of 20, 40 and 60 mM highly limited fungal growth of Colletotrichum in vitro conditions. The application of CaCl₂ solution could inhibit anthracnose lesion length of Cavendish banana variety, but its efficacy did not prolong until 6 DAI. In general, the good results were obtained from the 20 mM CaCl₂ in almost all the studied assays. Management of rot diseases on fruits by employing 20 mM CaCl₂ could be suitable to replace the current hazardous agro-chemicals.