Pre-service biology teachers (PBTs) are required to apply for information and communication technologies (ICTs) in their learning and professional teaching in the future. This study aims to investigate the applying need of ICTs for learning among PBTs, in Vietnam. A cross -sectional survey was conducted on 106 PBTs in Can Tho University Vietnam. Most of the PBTs (84.9%) were at moderate ability level of applying ICTs in their learning. PBTs have high percentage of advantaged skills in using word processing and email. The attitude towards appying ICTs in learning of PBTs is positive. Applying ICTs for learning among PBTs in Vietnam is not high. However, applying ICTs for learning, not only helps PBTs enhanced quliaty of teaching, but also helps them to futher learning in advance in the future. Future intervention should be conduted to increase the ability of applying ICTs, to improve the quality in learning, during teacher education training program for PBTs in Vietnam.