benh ph6 bi~n tren ga, gay thiet hai kinh t~ dang k6 cho nha chan nuoi i~t la tren ga gio (giam tang trong) va ga de (giam san hrong va chAtIUQ'ngtnrng), Khong nhu benh cum gia dm cao hay benh Newcastle, ga benh VPQTN thirong ty l~ chet thap, khong co trieu chirng d~c tnrng, thuong a th6 in nen rAt kho phat hien, Do do viec thi~t k~ c~p m6i d~c hieu trong chan doan va dinh chung vi-rut gay benh . - tren ga la rAt dn thi~t va hiru ich. Qua thiet k~ c~p m6i ill gene S1 bang Primer BLAST (NCBI), cap m6i -C CAGGA(T/C)TGTGC(AlG)TGGTGG-3' (m6i xuoi), 5'-AC(AlC)TCTTGTGC(T/G/A)GTACCATT-3' (rndi co dQd~c hieu cao trong chan doan phat hien va dinh chung vi-rut gay benh VPQTN tren ga. TYl~ m6i xuoi va pIQ'c co th6 phat hien cac chung tham chi~u l~n hrot la 79% (26/33) va 82% (27/33). Sau khi thuc hien phuong T-PCR sir dung cap m6i duoc thiet k~ co th6 phat hien diroc cac chung vi-rut nhom Massachusetts, QX-like, 'ii bien chung a Vi~t Nam. . css« doan, dinn danh, ga, vi-rut viem phi qudn truyen nhiem, thiil ki cap m6i