The results of study about medicinal plant resource of Khmer in Bay Nui area of An Giang province has classified 356 species belonging to 270 genera of 101 families in 3 phylla using for making medicine. In which, there were 8 species which are listed in the “Vietnamese Red Book” (2007) and the Government Decree 32/G/2006. The major life form of the medicinal plants to be surveyed was the grass group with 147 species, making 41.29%. The medicinal plants to be surveyed were arranged in six kinds of biotopes, in which the most acheivement was in the home garden, the orchard with 246 species, making up 69.10%. The medicinal plants to be surveyed can prevent and treat 21 disease groups.