The objective of the research was to screen actinomycetes able to control brown spot disease on rice caused by Bipolaris orryzae. Antagonistic efficacy evaluation of 8 selective antifungal actinomycetes isolates against B. oryzae fungus was laid out with five replicates. The result indicated that TG4.3 isolate showed higher stabler antagonistic ability with radiuses of inhibition zones is 6.6mm and antagonistic efficacy is 56.23% at 5 days after co-culture. On the other hand, the ability of inhibiting mycelia grownth of B. oryzae by extract of Actinomyces was examined with 5 replications. The result found that the extract of TG4.3 isolate inhibited mycelia grownth with lowest diameter of mycelia grownth is 32.0mm and were different significant compare with the other treatment at 6 days after testing.