Three actinomyces isolates that have inhibit growth of Fusarium solani cause yellow leaf and root rot on citrus are collected and isolated from citrus fields in Long My district, Hau Giang province (LM6 and LM25) and Lai Vung district, Dong Thap province (LV74) of the Mekong delta in 2017 (Le Minh Tuong et al., 2018). In this research, the these actinomycete isolates were identified based on morphological characteristics of cultured colony on the ISP mediums and their biochemical characteristics. In addition, the these isolates were also identified based on the 16SrRNA gene sequence. The results showed that LM6 and LM25 isolates are straight form of spore-bearing mycelium and spore chains; LV74 isolate’s spore-bearing mycelium belongs to straight form, spore chains with hooks form; surface spores was smooth with three isolates. The colors of substrate of the LM6 isolate belongs to red group, LM25 isolate belongs to light yellow group and LV74 isolate be long to white group. The LM6 and LM25 isolates can product melanin pigment, the LV74 isolate can’t product melanin pigment and all isolates have ability to product extracellular enzymes such as protease, lipase, amylase. Comparison of the 16S-rDNA gene sequence with existing on Gene bank indicated that LM6 isolate showed 99% similarity with Streptomyces capoamus isolate, LM25 isolate showed 100% similarity with Streptomyces bacillaris isolate and LV74 isolate showed 99% similarity with Streptomyces lavendulae isolate. The results of this study will be a basis for further researchs, contributing in applications of actinomycetes as biocontrol to yellow leaf and root rot disease on citrus.