Recently, anthracnose disease on lotus becomes popular at many lotus growing areas in the Mekong Delta of Vietnam, especialy in rain season. Identification of causal agent is neccessary for effective disease managment. Therefore, the objective of this research was to classify Colletotrichum species caused anthracnose disease on lotus in some provines of the Mekong Delta. There are 15 anthracnose fungi isolates were collected in Dong Thap, Can Tho, Hau Giang, Vinh Long, Bac Lieu  and Ca Mau province. The morphological, biological characteristics and pathogenicity were examined in Laboratory of Plant Protection Department, Collegeof Agricultureand Applied Biology, CanthoUniversitybased on the genera and species classification criteria of Barnett and Hunter (1998), Sutton (1980), Simmonds (1965), Mordue (1971), Swart (1999), CABI (2003) and other major documents. Among 15 isolates collected there were 5 isolates identified as Colletotrichum acutatum (1 isolate), Colletotrichum lagernarium (1 isolate), Colletotrichum gloeosporioides (3 isolates). Especially, Colletotrichum acutatum was newly detected as the agent of anthracnose disease on lotus. Moreover, results also showed that all fungal isolates are ability to infect and cause typical symtoms on different parts of the Nelumbo nucifera Gaertn (leaf, flower bud, seed-pod and seed).