Due to rapid urbanization, industrialization and economic growth, Can Tho City is facing several environmental issues such as air pollution and urban heat island (UHI). UHI causes relative warmth in city area compared to the surrounding rural areas. UHI causes more negative impacts on human health and comfort in big cities than in suburban areas. This research attempted to identify the spatial and temporal characteristics of UHI as well as to quantify the extent and intensity of UHI in Can Tho city using remote sensing data. The MODIS Land surface temperature remote sensing images of 12 months in 2014 were analysed to map the spatial extent UHI and calculate its intensity. The preliminary results show that UHI varies between day and night, in wet and dry season with different extent and intensity. The resulting isothermal map of Can Tho city enhances the efficiency of future urban planning and integrated environmental management.