Glossogobius sparsipapillus is one of the gobies that inhabits brackish and freshwater regions in the Mekong Delta. However, data on the biological parameters of this species population are limited. Therefore, the present study aims to provide the necessary knowledge on the fishing status of this goby living in freshwater (Cai Rang, Can Tho: CR) and brackish water regions (Long Phu, Soc Trang: LP). Data analysis results of 1379 individuals showed that males outnumbered females in LP (374 males: 288 females); the reverse case was found in CR (351 males: 366 females). There were five-six classes of length frequency, with the highest values between 8 and 12 cm (1122 individuals, 81.36%). The von Bertalanffy curve was L = 15.75(1
e
0.62(t + 0.30)) in CR and L = 15.85(1
e
0.83(t + 0.23)) in LP. Its longevity was 4.84 yrs in CR and 3.61 yrs in LP. The growth performance was 2.19 in CR and 2.32 in LP. The total, natural, and fishing mortalities were 2.17 yr
1 , 1.49 yr
1 , and 0.68 yr
1 in CR; 3.46 yr
1 , 1.68 yr
1 , and 1.78 yr
1 in LP, respectively. The fish stock has not been overexploited as E (0.31 in CR and 0.51 in LP) were lower than E0.1 (0.465 in CR and 0.652 in LP) 0.3.46 yr
1 , 1.68 yr
1 , and 1.78 yr
1 in LP, respectively. The fish stock has not been overexploited as E (0.31 in CR and 0.51 in LP) were lower than E0.1 (0.465 in CR and 0.652 in LP).