Citrus peel essential oil has considerable potential to be used as a direct agent against a wide range of Gram-positive and Gram-negative bacteria. The three citrus peel species used in this study included Citrus aurantifolia, Citrus latifolia, and Citrus hystrix. Essential oils were extracted on a pilot model and analyzed by gas chromatography-mass spectrometry (GC-MS). Gram-positive bacteria (Bacillus subilits, Listeria monocytogenes, Staphylococcus aureus) and gram-negative (Escherichia coli, Samonella typhimurium, Pseudomonas aeruginosa) were used to counteract the antibacterial effects of the samples. The yield of hydrodistillation distilled essential oil is C aurantifolia (1.15%), C latifolia (1.32%), and C hystrix (3.18%). The essential oil contains the main ingredient D-Limonene. The highest antibacterial activity against L. monocytogenes is Citrus latifolia essential oil. The main chemical composition of essential oils are β-pinene, D-limonene, γ-terpinene, terpinolene, α-terpineol, ... and the antimicrobial activity of the essential oil is affected by the variation of D-limonene. and β-pinene in essential oils.