An experiment was conducted to determine apparent ileal digestibility and total tract retention  of diets with inclusion of Tra catfish by-product protein hydrolysate by enzyme (HWWE) or by organic acid (HWWO), or with fish meal (FM) and soya bean meal (SB) suplementation in the diets for Noi local chickens. The digestibility was conducted with 24 male Noi chickens at 8 weeks of age and were placed in individual pens to collect excreta and ileal digesta. Four treatments HWWED, HWWOD, FMD and SBD were introduced to determine the apparent ileal digestibility (AID) and total tract retention (ATD) of nutrients using Chromic oxide (Cr₂O₃) as indigestible marker. After 4 days of colleting excreta, the chickens were killed to collect the ileal digesta. Results showed that, the AID and ATD of crude protein (CP) and ether extract (EE) were higher in HWWED and HWWOD diets compared to that of FMD or SBD diets. There were significantly increase total amino acid digestibility especialy isoleucine, leucine and lysine;  and ileal digestibility of isoleucine, leucine, lysine, methionine and threonine of catfish by-product protein hydrolysate than fish meal and soya bean meal in the diets.