Effect of soaking conditions (time, temperature and pH of the soaking solution) and germination conditions (aerobic and anaerobic) on the GABA content of IR 50404 and Jasmine 85 rice varieties was investigated. The rice was kept in water at room temperature (30± 2^oC)  and analyzed moisture content until it reached the optimum absorption. The optimum moisture content attainted after 6 h was 27.1 % and 32.7 % for IR 50404 and Jasmine 85 respectively. The optimum pH of soaking solution was pH 3 for IR 50404 and pH 2 for Jasmine 85 that gave the GABA content of 70,37mg/100g for IR 50404 and 78.50 for Jasmine 85. The germination under anaerobic conditions provided the highest GABA content which were 88.88 mg/100 g and 104.80 mg/100g for IR50404 and Jasmine 85 respectively after 30 hours.