Studies were carried out from June to December 2015 at the Biological control laboratory of Cantho university to evaluate the pathogenicity of fungus Colletotrichum gloeosporiodes on five kinds of citrus fruits by artificial inoculation with hurt fruit and non-hurt fruits mthods. The fungus was isolated from Citrus grandis var. Nam Roi and cultured Potato Dexose Agar (PDA). Results showed that disease syntoms appearred on hurt fruits, whole fruit rot and sporulation were earlier than non-hurt fruits of five citrus fruits.