The chemical examination on the sterms and leaves of Physalis angulata Linn, (Solanaceae) collected from Vinh Long province, in Vietnam, based on the results of in vitro cytotoxicity of ít extracts on cancer cell lines. From the ethyl acetate extract, five physalins were isolated: Physalin B (1), D (2), F (3), G (4) and I (5). The structures of these compounds were elucidated by analysing of NMR and MS data and by comparing with known spectral data.