Saltwater intrusion and increased soil salinity in the Vietnam Mekong Delta are reducing cultivated rice grain yield. The element silicon is known to improve the salt tolerance of rice grown in saline soils. This study evaluated the efficacy of silicate solubilizing bacteria (SSB) on the growth and yield of MTL480 rice cultivar grown in salt-affected soil under greenhouse conditions in two consecutive seasons. Five SSB, Ochrobactrum ciceri TCM_39 (TCM_39), Microbacterium neimengense MCM_15 (MCM_15), Klebsiella aerogenes LCT_01 (LCT_01), Citrobacter freundii RTTV_12 (RTTV_12), and Olivibacter jilunii PTST_30 (PTST_30)were isolated from bamboo, sugarcane, rice planted soils, earthworm intestine, and earthworm feces as bacterial sources for this study. Results showed that soil treatments inoculated with SSB enhanced the salt tolerance capability and yield of rice plants considerably compared to non-inoculated treatments. Three SSB inoculated treatments-TCM_39, RTTV_12, and a mixture of all five SSBs had significantly higher levels of the number of silicate solubilizing bacteria in soil and increased soluble silicate concentration in soil, silicate content in rice stem, K+/Na+ ratio in the rice stem and filled rice grain weight per pot compared to other treatments including the positive control (100% NPK recommended). Moreover, soil soluble silicon concentration strongly correlated with other soil parameters. This suggests that these five bacteria have a high potential for bio-product development to protect rice when grown in salt-affected soils.