This study was conducted at the Faculty of Agriculture and Applied Biology, Cantho University aims at selecting the best Bacillus strains effective for the control of chili anthracnose caused by Colletotrichum gloeosporioides. Results of in vitro evaluation selected three strains of Bacillus-101, Bacillus-PH1.5et and Bacillus-PH4.7t with high antagonistic efficiencies of 63.38%, 62.50% and 59.96%, respectively, at 9 days post-test, equivalent to Score 250EC. Under in vivo conditions using detached fruit puncture method inoculation with conidial suspension of C. gloeosporioides (5 x 10⁵ conidia ml^-1 water), in laboratory conditions or on growing plants (“Sừng vàng Châu Phi”cultivar) under net house conditions, Bacillus-101, Bacillus-PH1.5et, Bacillus-PH4.7t and B. amyloliquefaciens were able to control chili anthracnose disease with the same efficacy as Chubeca 1.8DD and Score 250EC