This study aimed to evaluate the level of contaminated coliforms and E. coli on sandwiches sampled in Ninh Kieu District, Can Tho city, Vietnam, and further testing of antibiotics susceptibility of E. coli. The results showed that the contaminations of coliforms on collected sandwiches taken at school, hospital, and market areas were 5.3±1.3, 5.4±0.8 and 5.3±0.6 log CFU/g, respectively; while the contaminations of E. coli at these areas were 3.3±1.5, 3.6±1.0 and 3.3±1.0 log CFU/g, respectively. 17/23 (73.9%) of E. coli isolates from the sandwiches were multi-antibiotics resistant. The high resistance was observed to ampicillin (87%) followed by tetracycline (60.9%), cefotaxime (56.5%), chloramphenicol (43.5%), and sulfamethoxazole/trimethoprim (39.1%). As the sandwiches are popular and favorite street food, it is suggested that the safety and the quality of the sandwiches should be controlled to ensure public health.