Title: Efficiency of Bacteriophage in Controlling of Rice Bacterial Grain Rot Caused by Burkholderia glumae in The Field Conditions

Bacterial grain rot on rice caused by Burkholderia glumae is an important disease in Vietnam and other
tropical countries. Phage therapy is considered as a potential agent for controlling the bacterial disease. The aim of the study on the evaluation of phage cocktail is to prevent rice bacterial grain rot in Vinh Long province in two rice growing seasons (Winter – Spring and Early Summer- Autumn). These experiments were followed by randomized complete block design (RCBD) including four treatments (i. e. phage cocktail (10⁸ pfu/ml), phagecocktail (10⁷ pfu/ml), oxolinicacid, and control) with four replications. In the Winter – Spring season, there were three treatments such as phage cocktail (10⁸ pfu/ml) and (10⁷ pfu/ml), oxolinic acid gave a lower rate of infected grains and AUDPC than the control. Moreover, phage cocktails (10⁸ pfu/ml) showed the highest actual yield compared to others. Similarly, in the early Summer- Autumn season, phage treatment and oxolinic acid treatment showed a significantly lower rate of infected grains compared to the control. Especially, the phage cocktail (10⁸ pfu/ml) was the best treatment in disease reduction and plant yield protection.
Keywords: Bacteriophage, biological control, Burkholderia glumae, phage cocktail, phage titer.