This research focuses on the isolation and characterization of eight natural compounds from the ethyl acetate extract of Scoparia dulcis L. collected in An Giang province, Vietnam. Six flavonoid compounds: apigenin (1), luteolin (2), baicalein (3), oroxylin A (4), oroxylin A 7-O-βD-glucoside (5) and vitexin (6) along with two nitrogen-containing compounds: 2,4-dihydroxy1,4-benzoxazin-3-one  (7) and uracil (8) were successfully isolated via the methods of natural product extraction. The structures of these compounds were elucidated base on 1D-NMR and 2D-NMR spectral data as well as MS and compared with those in the authentic reports. This study contributed new results to the phytochemical examination of Scoparia dulcis Linn. growing in Vietnam with the presence of the first isolated compounds as (3), (4), (5), (7) and (8).