Mixing two or more essential oils (EOs) may increase effectiveness of mango anthracnose control. In this study, EOs from a wide range of plant materials were hydro-distilled and chemically characterized. EOs were tested against in vitro growth of Colletotrichum gloeosporioides. EOs with the highest antifungal toxicity were selected for the interactive experiments. The results showed that orange had the highest extraction yield (4.94%), followed by lemon, pomelo, citronella, turmeric, ginger, lemongrass (LG), cinnamon bark (CB), cinnamon leaf (CL) and basil. Trans-cinnamaldehyde was the most abundant compound of CB (91.3%) and CL (94.1%) EO. Limonene was principle constituent of orange (97%), pomelo (96.1%) and lemon (67.2%). Citral (78.4%), methyl chavicol (76.1%), linalool (72.7%) and geraniol (69.1%) were major chemicals of LG, basil, coriander and citronella EO, respectively. Dominant compounds of ginger, turmeric and peppermint EO were zingiberene (23.8%), ar-turmerone (33.9%) and menthol (40.8%), respectively. LG, CB and CL EO had the strongest antifungal activities against fungal growth, and achieved minimum fungicidal concentration (MFC) of 2, 0.8 and 0.4 µl/ml, respectively. Mixture of LG (0.8 µl/ml) with CB (0.025 µl/ml) or CL (0.05 µl/ml) showed synergistic effect against pathogen with MFC at lower concetrations than that of EOs alone. Similarly, EO mixtures had synergistic effect on control of mango anthracnose. CL and CB EO alone as well as their mixture with LG EO were more effective in controlling mango anthracnose than commercial fungicide. EOs alone and their mixture had no or negligible effects on mango quality. In short, CL, CB alone and their mixture with LG EO can be commercially used as biofungicides to control mango anthracnose.