Vocabulary learning plays an important role in language learning. To many language learners, lack of vocabulary is one of the biggest obstacles that prevent them from mastering the language. Moreover, there have been a lot of studies conducted to find out the way to help language learners acquire and retain learned vocabulary. Among them, PowerPoint is suggested by many researchers as one good way to help learners learn vocabulary retention through reading comprehension. The purpose of this study was to investigate the effect of PowerPoint on vocabulary retention in reading comprehension of EFL (English as a Foreign Language) first-year students at Long An University of Economics and Industry (DLA), Long An Province, Vietnam. Besides, this study investigates learners’ attitudes towards the use of PowerPoint in learning vocabulary. The participants were 60 non-English major students at DLA. Two instruments were (1) the tests on English vocabulary, (2) the questionnaire on the participants’ attitudes towards the use of PowerPoint in teaching and learning vocabulary, were investigated. The results demonstrated a significant difference in learners’ vocabulary retention: the participants in the experimental group remembered vocabulary longer than those in the control group. The study showed that the learners had positive attitudes towards the use of PPT (PowerPoint Presentation) in learning vocabulary retention.