Five extracts from the aerial parts of Cayratia trifolia (L.) Domin (n-hexan, chloroform, ethyl acetate, methanol and methanol-H2O (80:20)) were screened for antimicrobial activity against 8 microbial strains by broth microdilution assay. The chloroform extracts exhibited the antifungal activity against F. oxysporum, S. cerevisiae and C. albicans with the MIC value of 200-400 µg/mL. Phytochemically, from the chloroform extracts, three following compounds were isolated: Stearic acid (1), lutein (2) and b-sitosterol-3-O-6’-stearyl-b-D-glucopyranoside (3). Their structures were confrmed by ESI-MS, NMR in reference to the literature.