In this study, chitosan nanoparticles (CTS NPs) have been suscessfully combined with Carica papaya leaves (CPLs) extract for 30 min at room temperature. The prepared chitosan nanoparticles (CTS NPs)/Carica papaya leaves (CPLs) extract have been characterized by UV-vis, FTIR, SEM and TEM. The result showed these CTS NPs/CPLs extract obtained with the average particle size of ~20-50 nm. Moreover, the synthesized CTS NPs/CPLs extract also showed efficient their anti-organism activity for the killing of yellow snails occurred completely after 24 h. The presence of a small powder amount (500 mg) of chitosan nanoparticles (CTS NPs)/Carica papaya leaves (CPLs) extract in (5 L) water was enough to inhibit and kill yellow snails completely only after 24 h.