A simple and eco-friendly approach has been successfully developed for the preparation of chitosan nanocomposites films loaded with silver nanoparticles using kumquat extract as a biological reducing agent. The chitosan/silver nanocomposites (CTS/Ag NCPs) films were prepared from chitosan/silver nanocomposites solution and dried for 14 h at 70oC in a vacuum oven with the pressure of 0.03 Mpa. The morphology and characterization of CTS/Ag NCPs films have been also determined by FTIR, XRD, and SEM. The UV-vis spectroscopy and TEM image indicated that synthesized chitosan/silver nanocomposites have spherical shape with their uniform dispersion and their average particle size of about 20-30 nm. The prepared CTS/Ag NCPs films showed their great antibacterial activity on Staphylococcus aureus (S. Aureus) and Escherichia coli (E. coli). Therefore, this eco-friendly method that would be used for the preparation of chitosan/Ag nanocomposites films could be competitive and alternative to the existing ones. Moreover, CTS/Ag NCPs films would have their high potential for biomedical applications (i.e, medical tape, burn treatment, etc.), opto-electronics and medical devices in the current time and in the future.