The presence of biofilms in milk industry may be lead to deterioration in quality of dairy products and a risk to
consumer health. The main purpose of the article is to evaluate biofilm formation ability in the 96-well
microtiter plate of bacterial strains isolated from raw milk samples of cow-milk farms in BenTre province,
Mekong Delta - Vietnam and identify isolates as strong biofilm formers. A total of 10 bacterial isolates is
isolated from 8 raw milk samples and studied for their capabilities to form biofilm. All the isolates had biofilmforming
ability. 3, 1 and 6 of them can be classified as strong, moderate and weak biofilm producers. Among
the strong biofilm formers, the BT17, BT119 isolates were 100%, 98% of identity Pseudomonas oryzihabitants
strain 2.1, Serratia marcescens NPK2 2 20 respectively. The BT31 isolate had the strongest biofilm ability,
extracellular proteolytic and lipolytic enzymes activities and was Aeromonas allosaccharophila BT31.