One of the most important economic sectors contributing to the industrial growth in Vietnam is aquatic products processing industry, especially focusing on the seafood export as shrimp. More and more Vietnamese companies have applied Lean Manufacturing to enhance the product quality and to reduce the production cost, which is called non-value activities for the system production. The aim of this paper is to implement Value Stream Mapping (VSM) in production line at Ocean Shine Company, a fundamental tool of Lean manufacturing that involves constructing an overview of the whole production system and identifying wastes. As a result, some suggestions supporting to reduce wastes and inventory time of the value stream production line will be applied in the short term. Focusing on the current and the future state VSM was established. In addition, ARENA software has been used to build up two simulation models based on two VSMs. The application results in the enterprise are also presented specifically.