Water hyacinth (WH) is promising biomass for renewable energy production when combined with livestock manure. However, previous studies have only investigated biogas production at low loading rates. In this study, we investigated the effects of various volatile solid (VS) loading rates (0.5%, 1.0%, 1.5%, 2.0%, and 2.5%) on biogas production using WH in batch bioreactors. pH, temperature (oC), biogas production volume in five days (L), the total volume of biogas production (L), biogas yield (L/kgVSadded), and methane concentration (%) were measured over 45 days. The results showed that pH and temperature were suitable for methane production. The VS loading rate of 2.5%VS achieved the highest volume of biogas production in five days, while the loading rate of 0.5%VS recorded the lowest volume. Although, the 2.5%VS treatment had the highest cumulative biogas volume, and was significantly different from the other treatments (p=0.001