Vibrio parahaemolyticus is the causative agent of shrimp diseases, the most serious of which is acute hepatopancreatic
necrosis disease (AHPND). Bacteriophage is a virus of bacteria that can parasitize and destroy bacteria, so it is considered a
potential alternative to antibiotics. The study was carried out to evaluate the treatment ability of bacteriophages on Litopenaeus
vannamei after being infected by V. parahaemolyticus B4XOT2.2 isolated from the bottom mud of shrimp ponds infected
with AHPND. The study used the histopathological survey method on shrimp at all three ages: postlarvae, adult shrimp at the
ages of 30–45 days, and 55–60 days old, which were arranged into three treatments: healthy shrimp, diseased shrimp, and
bacteriophage-treated shrimp. The results showed that: the concentration of Vibrio spp. in bacteriophage-treated treatment
was decreased in all three groups (postlarvae, shrimp at the ages of 30–45 days, and 55–60 days old) from 3,7x103 CFU/
mL to 2,2x102 CFU/mL after two days, from 4,6x104 CFU/mL to 3,3x102 CFU/mL after two days and from 4,6x104 CFU/
mL to 5,6x103 CFU/mL after three days, respectively. The pathological signs and histological features of the infected shrimp
samples were similar to those typical of acute hepatopancreatic necrosis disease and recovered in shrimp tissue after being
treated with bacteriophages such as the hepatopancreas was also darker, the intestines gradually filled, no hematoma around
the tubules were found, in the lumen of the tube reduced sloughing cells and blood cells, no melanization was observed.