Doing research on folk literature in relation to folklore has been used effectively by folklore experts around the world. Recently, in Vietnam, this research direction has also been becoming more popular and gaining practical values in finding the material and spiritual cultural values, as well as behavioral cultural values with the natural environment which are hidden in folk literature, including folk-songs. Folk-songs in the Lower Mekong Delta regions are spiritual products of people living in the land with interlacing rivers and canals. It is the characteristic that contributes to creating specific features for folk-songs of new land. By applying some methods such as statistics, analysis, synthesis, and comparison, this article presents some features of river culture in the folk-songs of the Lower Mekong Delta regions to distinguish them from Vietnamese folk-songs in general. Keywords: folk-songs, Mekong Delta, river cultur