Background and Objective: Lasia spinosa L. Thwaites is an herbaceous perennials plant that is well- known for its multiple functions in terms of pharmacological effects. This study aimed to identify the capability to against free radicals and enzymatic glycosylation in vitro to examine its oxidative stress inhibition, thereby discover an innovative diabetes therapy of the stem extracts of Lasia spinosa L. Thwaites. Materials and Methods: The fractions were first used to determine the phytochemical constituents, the total content of phenolic and flavonoid. Antioxidant activities were evaluated by using various assays including DPPH, ABTS, Total Antioxidant Capacity (TAC) and Reducing Power (RP). The findings through "-amylase and "-glucosidase inhibition assays finally demonstrated the antidiabetic capabilities of the fractions. A one-way analysis of variance experiment was carried out by applying SPSS 18.0 software. The significant differences between means which were defined by Student’s t-test and p-valuesResults: The result revealed that the extract of ethyl acetate fraction of Lasia spinosa L. Thwaites stems presented high levels of phenolic and flavonoid compounds. The ethyl acetate extract also had an antioxidant activity with the most efficient EC50 value, compared to the remaining in DPPH, ABTS, TAC and RP assays. Further examination with enzymatic glycosylation inhibition showed a remarkable IC50 value in the anti-diabetes assay of ethyl acetate fraction. Conclusion: The present study has given primary evidence for Lasia spinosa L. Thwaites stem extracts to be considered as a therapeutically valuable agent for the treatment of diabetes mellitus and oxidative stress co-morbidities.