The study aimed to assess the variation of surface water quality in the water bodies in Phong Dien district, Can Tho city, Vietnam. Data were collected in 2019 at 5 locations in the district, namely My Khanh (MK), Phong Dien (PD), Giai Xuan (GX), Truong Long (TL) and Vam Sang market (VS). The samples were collected in the dry and rainy seasons, each collection period was performed at low and high tide. Surface water quality was evaluated using 12 water quality parameters belonging to the group of physical, biological and chemical parameters including pH, temperature, dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids (TSS), ammonium (NH₄⁺-N), nitrite (NO₂^--N), nitrate (NO₃^--N), orthophosphate (PO₄^3--P), iron (Fe), coliform. Water quality was compared with the national technical regulation on surface water quality QCVN 08-MT: 2015/BTNMT (column A1). The results showed that the study area had organic pollution (high BOD and COD), microorganisms. Nutrient parameters were still within the allowable limits of QCVN 08-MT: 2015/BTNMT. The concentration of nutrients and organic matter in the dry season at low tide was higher than that in the rainy season in high tide. Coliform were only detected at low time sampling period. The source of organic and microbiological pollution was mainly from domestic and animal husbandry wastes discharged directly into the water environment by the households. The results revealed that surface water quality in the water bodies in Phong Dien district, Can Tho city was no longer suitable for domestic needs. The environmental managers need urgent solutions, for example solid waste and wastewater collection and treatment systems, to solve the problem of surface water pollution.