Chitosan nanoparticles (CTS NPs) loaded Houttuynia cordata leaves (HCLs) extract possess highly antibacterial activity. The aim of the present study was to investigate in combination of chitosan nanoparticles (CTS NPs) and Houttuynia cordata leaves (HCLs) extract with reaction time of 30 min at room temperature. The prepared chitosan nanoparticles (CTS NPs)/Houttuynia cordata leaves (HCLs) extract have been characterized by UV-vis, FTIR, SEM and TEM. The obtained result showed these CTS NPs/HCLs extract have been gained with the average particle size of ~10-25 nm. Moreover, the synthesized CTS NPs/HCLs extract also showed efficient their antibacterial and antifungal activity on the preservation of Oranges. The result showed that the presence of a small powder amount (500 mg) of chitosan nanoparticles (CTS NPs)/Houttuynia cordata leaves (HCLs) extract in (2 L) water was enough to inhibit against bacterial and fungal on preservation of oranges due to the vitamin C content in the observed orange sample still remaining about 85.36% after 19 days of storage at room temperature as compared to that of original orange sample. Thus, it would be highly potential material to be used in biomedical, antimicrobial and antifungal applications in future.