In this research, a set of experiments was carriedout for identifying the optimum conditions of independent 

variables affecting phenolic content extraction efficiency and antioxidant activity of soybean seeds  (Glycine max L.).They 

included the use of different organic solvents (methanol, ethanol and acetone); concentrations of solvent (40, 50, 60, 70, 80,

and 90 v/v, %); the soybean–to–solvent ratio (1:4,  1:6; 1:8 and 1:10) and the number extraction cycles(2, 3 and 4); the 

extraction time (2, 3 and 4 hours) and the temperature (30, 40, 50 and 60oC). The extraction abilities of phenolics manifested in forms of total phenolic and total flavonoid contents (TPC and TFC) as well as the antioxidant activityby 1,1–diphenyl–2–

picrylhydrazyl radical scavenging (DPPH) were used  as assessment indicators. Generally, high extraction yield was obtained 

using aqueous acetone 70% as solvent; the most suitable soybean–to–solvent ratio was 1:6 for 3 cycles  of extraction. The 

extraction yield could further be increased using aprolonged time of 3 hours at the temperature of 40°C.