The objectives of this study were analysis of the secondary metabolite products from extract of marine Bacillus subtilis strain HD16b which isolated from sponges at Ha Tien Sea, Kien Giang province, Vietnam. Eight bioactive compounds were identified in the organic solvent ethyl-acetate. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular weight and molecular formular. GC-MS analysis of Bacillus subtilis strain HD16b revealed the existence of the phenylacetic acid, pentadecanoic acid, pyrrolo[1,2- a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), n-Hexadecanoic acid, cis-10-Heptadecenoic acid, octadecanoic acid, didemnin B and 1,2-Benzenedicarboxylic acid, diisooctyl ester