The study was conducted to investigate the use of chemicals, drugs, and disease occurrence from frog farming in Dong Thap province. Data were collected through a questionnaire from a random sample of 30 frog farmers from January to April 2020. The results showed that the common diseases were pus in liver (66.7%), hemorrhage (66.7%), and digestive tract problems (flatulence, abdominal intestine, 40%). Farmers used antibiotics to treat diseases and chemicals to treat parasitic infection without knowledge on pathogens. Commonly used antibiotics were oxytetracycline (53.3%), doxycycline (63.3%), amoxicillin (53.3%), florfenicol (36.7%), and sulfamethoxazole/trimethoprim (46.7%). In addition, other antibiotics such as tetracycline, enrofloxacin, ciprofloxacin, rifampicin were used in some households. Farmers used iodine (26.7%), copper sulfate (20%), and BKC (16.7%) as disinfectants. There was no antibiotic residues test prior to sell to traders. It is necessary to support farmers in knowledge on diseases, drugs/chemicals use, and safety in the use of drugs and chemicals.