The proximate composition and amino acid profiles were evaluated in seven common green seaweeds (Cladophoraceae) from the Mekong delta of Vietnam, namely Cladophora sp., Cl. socialis, Cl. crispula, Cl. patentiramae, Rhizoclonium sp., Rh. kochianum, and Chaetomorpha linum. Results showed that nutritional composition of green seaweed varied among species. Seaweed proximate contents varied in the ranges of 11.49-19.29% for protein, 0.81-2.29% for lipid, 20.87-24.75% for ash, 39.87-54.19% for carbohydrate, 0.67-1.58% and 0.08-1.39% for calcium and phosphorus, respectively. The highest contents of essential amino acids were observed for isoleucine, followed by leucine, especially methionine was relatively high. For the non-essential amino acids such as aspartic acid, serine and lysine were more dominant than other individual amino acids. Overall, these investigations provide nutritional values of autochthonous green seaweeds Cladophoraceae in the Mekong delta. These seaweed can be used as feed ingredients in aquafeeds.