The study was conducted to evaluate the effects of age and levels of vitamin E supplementation in the diet on sperm quality of crossbred buck rabbits (New Zealand white × Local) in the Mekong Delta. Twenty-four crossbred buck rabbits in 2 age groups, 5-6 months old (12 young bucks) and 9-10 months old (12 mature bucks) were supplemented with vitamin E in a diet of 0, 40, 80, and 120 mg/kgDM feed (E0, E40, E80, and E120), respectively. The experiment was carried out for 12 weeks and recorded the quality of buck rabbit sperm once a week. The experimental results recorded that buck rabbits with an age range of 9-10 months had a sperm concentration of 414×106/ml and the sperm motile rate 55.6% better than the bucks with an age range of 5-6 months with sperm concentration of 284×106/ml and sperm motile rate of 47.2% (P0.05). The experimental results showed that buck rabbits aged 9-10 months and supplemented with 40mg of vitamin E were suitable for sperm extraction in the farming conditions of the Mekong Delta.