This study assessed antagonistic capacity of various indigenous microorganisms (IMO) collected from different cropping systems within Soc Trang province against plant pathogenic fungus including Fusarium oxysporum and Rhizoctonia solani. Biocontrol activity of fifteen collected IMOs was investigated on PDA agar media for 5-7 days under laboratory conditions with three different scenarios. IMO and pathogenic fungus were incubated at the same time and IMO was introduced before and after inoculation of plant pathogenic fungus. The results illustrated that all surveyed IMOs were found to have highly potential biocontrol against two plant pathogenic fungi to different extents and IMOs which were introduced before the inoculation of pathogenic fungi showed the highest efficiency in biocontrol of plant pathogen. Particularly, four out of fifteen IMOs which were collected from bamboo, shallot, grapefruit and guava farms showed their highest antagonistic efficacy on Fusarium oxysporum biocontrol by completely decaying fungal hyphae of this fungal strain after seven incubation days. For Rhizoctonia solani, all IMOs displayed highly antagonistic ability with inhibitory percentages varying between 52.96% and 92.59% after two days. The antagonistic functions of all collected IMOs could be exploited for plant protection from plant-pathogenic fungus.