The aim of this study was to assess antagonistic capacity of various indigenous microorganisms (IMO) collected from different cropping systems within Soc Trang province against plant pathogenic fungus including Fusarium oxysporum and Rhizoctonia solani. Bio-control activity of fifteen collected IMOs was investigated on PDA agar media for 5 - 7 days under the laboratory conditions with three different scenarios. IMO and pathogenic fungus were incubated at the same time and IMO was introduced before and after inoculation of plant pathogenic fungus. The results illustrated that all surveyed IMOs were found to have highly potential in bio-control against 2 plant pathogenic fungi with different extents and IMOs which were introduced before the inoculation of pathogenic fungi showed their highest efficiency in bio-control of plant pathogen. Particularly, four out of fifteen IMOs which were collected from bamboo, shallot, grapefruit and guava farms showed their highest antagonistic efficacy on Fusarium oxysporum bio-control by decaying completely fungal hyphae of this fungal strain after 7 incubation days. For Rhizoctonia solani, all IMOs displayed their highly antagonistic ability with inhibitory percentage varied between 52.96 % and 92.59% after 2 days. The antagonistic functions of all collected IMOs could be exploited for plant protection from plant-pathogenic fungus.