Article info.

ABSTRACT

Received date: 05/07/2016

Accepted date: 30/03/2017

In this study, the extraction of phenolic compounds from Pouzolzia Zeylanica L. Benn was conducted by using pure water as a solvent. The optimal conditions for the extraction of three phenolic compounds such as polyphenols, flavonoids and tannins were determined by using response surface methodology (RSM). A central composite design (CCD) was applied to investigate the effects of three independent variables, namely the ratio of water-to-dried material (20:1 to 30:1, v/w), temperature (70 to 90°C) and time extraction (20 to 40 minutes). The dependent variables were total polyphenol content (TPC), total flavonoid content (TFC) and tannin content (TC). A second-order polynomial model was used for predicting the response. Optimized conditions for bioactive compounds extraction, water-to-dried material ratio, time and temperature extraction were 27 (v/w), 30 minutes and 81°C, respectively. The experimental values agreed with predicted values within a 95% confidence interval. Total polyphenol, flavonoid and tannin content extracted by these optimized conditions were achieved (921 mgGAE/100g dried material (DM), 563 mgQE/100g DM and 643 mgTAE/100g DM, respectively).

Keywords

Extraction, phenolic compounds, pouzolzia zeylanica L. benn, optimization, response surface methodology

Cited as: Tan, N. D., Viet, L. Q., Thanh, V. T., Thuy, N. M., 2017. Optimization of polyphenol, flavonoid and tannin extraction conditions from Pouzolzia zeylanica L. benn using response surface methodology. Can Tho University Journal of Science. Vol 5: 122-131.