This study was to discover a new source of lipases for potential use to meet the growing demands for enzymes in commercial applications. In this connection, the appropriate conditions for enzyme lipase extracted from the offal of snakehead (Channidae) were investigated. Homogenized offal with different ratios was extracted using various buffers and pH adjusted solutions at buffers and pH adjusted solutions at different temperatures and time. Based on the result, the best lipase extraction effect achieved when using phosphate buffer solution (pH 6.0) with the ratio of material and extraction solvent was 1:4, extraction process during 3 hours at 50ºC. In this optimal condition, the highest enzyme activity obtained was 71.28 ± 1.92 U/g of dry material. The study results have demonstrated the potential acquisition lipase from offal of snakehead fish, increase economic efficiency and reduce waste in the environment.

Keywords: extraction temperature, extraction time, phosphate buffer, lipase, snakehead fish offal