Desulfovibrio spp. bacteria pose significant challenges in shrimp aquaculture by producing hydrogen sulfide (H₂S), which depletes oxygen levels and hinders shrimp growth. The increasing prevalence of antibiotic-resistant bacteria necessitates the exploration of alternative control methods. This study investigates the potential of bacteriophages as a biological solution to inhibit Desulfovibrio spp. in shrimp ponds. Six bacteriophage strains (ST231, ST22, φMix 1, φS8, φS9, and φMix 2) were evaluated using plaque assays at three time points (3, 6, and 18 hours). Results demonstrated that most bacteriophages significantly reduced bacterial density over time, with ST22 showing the most substantial inhibition (pDesulfovibrio spp., specifically Deltaproteobacteria and Desulfobacteraceae. The study also found that bacteriophage treatment led to decreased H₂S and NH₃ levels and increased dissolved oxygen in simulated pond environments. Morphological changes in bacterial colonies post-treatment further supported the phages’ inhibitory effects. These findings suggest that bacteriophage ST22 is a promising biological alternative to antibiotics for preventing and controlling diseases caused by Desulfovibrio vulgaris in shrimp aquaculture, though further research is needed to assess long-term ecological impacts and efficacy in complex pond ecosystems.