One new calamenene sesquiterpene glycoside, (1R,2R,3S,4S)-4-isopropyl-1,6-dimethyl-1,2,3,4-
tetrahydronaphthalene-2,7-diol 3-O-β-D-glucopyranoside (1), along with five known compounds including
betulinic acid (2), oleanolic acid (3), maslinic acid (4), daucosterol (5), and 6′-O-acetyl-β-daucosterol (6),
was isolated from the bark of Thespesia populnea. The n-hexane, dichloromethane, and ethyl acetate extracts
showed inhibitory effects against four strains of microorganisms, E. coli, S. aureus, A. niger, and F. oxysporum,
with MIC values of 50–200 μg/mL while the acetone extract only exhibited antifungal activity against
A. niger with MIC 50 μg/mL.