Three new earthworm species are described from Dong Nai Province, Vietnam, namely Metaphire malayanoides Nguyen & Lam sp. n., Metaphire grandiverticulata Nguyen & Lam sp. n., and Metaphire xuanlocensis Nguyen & Lam sp. n. Both M. malayanoides sp. n. and M. grandiverticulata sp. n. have four pairs of spermathecal pores in ventrolateral 5/6/7/8/9. However, M. malayanoides is distinguished by having two to eight pairs of genital markings located in intersegmental furrows, starting from 19/20; M. grandiverticulata sp. n. is characterized by having only one pair of genital markings between male porophores in xviii, and very large tube-shaped diverticulum. M. xuanlocensis sp. n. has one pair of spermathecal pores inside the spermathecal chambers in 7/8, accessory glands stalked and attached to the enlarged base of prostatic duct, and the presence of penial setae