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Bài báo - Tạp chí
229 (2024) Trang: 227-234
Tạp chí: TNU Journal of Science and Technology

The storage medium for liquid semen and storage time play an important role in maintaining and stabilizing sperm quality during storage. The
objective of the study was to determine the carbon source in the appropriate storage environment and optimal storage time to preserve the
best sperm quality. The study evaluated the role of three different carbon sources in the storage medium: glucose, fructose and sucrose. This study included 6 treatments corresponding to 6 types of storage media, each solution was repeated 8 times. After collection, semen samples were
diluted with storage medium at a ratio of 1:10, then the samples were stored at 15°C. The quality assessment according to specific time points
were 0 hours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours. Research results showed that sperm quality decreases with storage time.
The results of microscopic evaluation showed that, after 72 hours of storage, the best quality of goat sperm was recorded in Tris Citrate
Sucrose-2 medium. Specifically, the overall motility rate was 71.61%, progressive motility rate was 64.91%, sperm viability rate was 74.50% and
the membrane integrity was 57.59%. The results showed that sucrose improved sperm quality better than glucose and fructose, Tris Citrate
Sucrose-2 medium helped keep the best sperm quality and the appropriate storage time was 72 hours.

 


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