Staphylococci are one of the most commonly found opportunistic bacteria in animals and human. The objective of this study was to determine the prevalence of Staphylococcus spp. isolated from healthy and sick dogs visiting veterinary clinics in Ho Chi Minh city, Vietnam from January 2022 to December 2022. A total of 123 nasal swab samples were collected, including 40 samples from healthy dogs and 83 samples from dogs with respiratory symptoms. Staphylococcus spp. were isolated from 47.5% of healthy animals and 60.24% of dogs showing respiratory symptoms. The study showed that isolation rates from confined, semi-confined and free-ranging dogs were 75.86%, 54.84% and 40.63%, respectively. Furthermore, the prevalence was higher in dogs over one year old compared to younger dogs (64.10% and 42.22%, respectively). Several subspecies of Staphylococci, including S. aureus, S. pseudintermedius and S. epidermidis were identified, in which S. pseudintermedius accounted for the highest proportion (48.28%). The prevalence of S. pseudintermedius also varied across different age groups. Coagulase activity of the isolated Staphylococcus strains was also determined and indicated that coagulase-positive Staphylococci constituted 80.46% of the isolates. This study represents the first report on the prevalence of Staphylococci in dogs in Vietnam and reemphasizes the zoonotic significance of Staphylococcus at the interface between dogs and humans.