The preparation of CS/HA nanofibers was successful via a wet chemical method. Homogenous CS nanofibers were fabricated by an electrospinning technique. The CS nanofibers were cross-linked by GA before mineralization by the reaction of Ca(NO₃)₂ and (NH₄)₂NO₃.  The reaction with three cycles (C3) was optimized the time for the preparation of electrospun CS/HA nanofibers. The time of mineralization by using the wet process was about 3 hours. Meanwhile, the time of mineralization by using SBF treatment was at least 144 hours (6 days). HA XRD, SEM, FTIR, EDS analyses confirmed the formation of HA, which was similar to HA found in nature bone. The scaffolds of electrospun CS/HA nanofiber were suitable for bone cells.