This study aims at determining some biochemical characteristics and antifungal activities against 2 fungal plant pathogens, namely Phytophthora sp. and Rhizoctonia sp. from fractions of Annona glabra, Calophyllum inophyllum and Phyllanthus amarus. All extracts displayed high level of polyphenol, flavonoid and tannin compounds, while alkaloid compounds were lower among fractions. They indicated strong antioxidant activities through reducing power (Fe³⁺), DPPH radical scavenging and hydrogen peroxide (H₂O₂) assays. Antifungal abilities were carried out by agar well diffusion method at the concentration of 200 mg/mL with the inhibition zone ranged from 3.5 ± 0.5 mm to 6.3 ± 0.6 mm. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentraion (MFC) of Annona glabra extract sample 2 on Phytophthora sp. were 5 and 50 mg/mL while that of Calophyllum inophyllum extract sample 1 were 5 and 20 mg/mL. MIC and MFC values of Calophyllum inophyllum extract sample 2 on Rhizoctonia sp. were 5 and 25 mg/mL.

Keywords: antifungal activities, biochemical characteristics, fungal plant pathogens.