This study contributed to the variation of morphometric and meristic measurements of Glossogobius sparsipapillus, a target catching fish in the Mekong Delta. A total of 583 individuals (293 males and 290 females) were collected during dry and wet seasons (April 2019 to January 2020) at three studied sites from Vinh Hau, Hoa Binh, Bac Lieu to Dien Hai, Dong Hai, Bac Lieu and Tan Thuan, Dam Doi, Ca Mau. The analysis results showed that total length and body weight of this species varied with seasons and studied sites, but not genders. The variations of fish length and weight of males and females depended on seasons but not studied sites. The interaction of site and season variables influenced the change of fish length and weight. The results supplied additional knowledge for fish identification and ecological adaptation understanding in the study regions.