Antibiotics play an important role in aquaculture. However, abuse will leave a high residue of high levels of antibiotic residues that can endanger the environment by spreading of water to neighboring ponds and lakes. Therefore, it is important to control the residues of antibiotics in the water environment, especially on the water surface. For that we must have a system to collect and monitor residues of antibiotics in the environment. This paper proposes a simulation model to track antibiotic residues along the river with aquaculture sites based on the cellular automata theory. The results are based on data on antibiotic residues collected in Hau River, O Mon district, Can Tho City.